Identification and Detection of Dyeing Components in Counterfeit Cinnabar (Elutriation)

作者：Hailin RAN

摘要：[Objectives]This study aimed to analyze the dyeing status and dyeing components of counterfeit cinnabar(elutriation),in order to provide technical basis for cinnabar(elutriation)supervision.[Methods]The scarlet 808 in cinnabar(elutriation)was identified qualitatively using thin-layer chromatography(TLC)and high-performance liquid chromatography(HPLC),and the positive samples were confirmed by high-performance liquid chromatography-mass spectrometry(HPLC-MS).The conditions were as follows:column,Agilent Eclipse Plus C 18;mobile phase,acetonitrile-0.1%formic acid(85∶15);detection wavelength,520 nm;ion source,electrospray ion source;scanning mode,positive ion scanning;scanning range,100-700 m/z;cone voltage,3.5 kv;drying gas temperature,350℃;drying gas flow rate,12 L/min.[Results]The thin-layer chromatography spots were clear and separated well.Among the 13 batches of samples,12 batches were positive for scarlet 808.[Conclusions]The counterfeit cinnabar(elutriation)currently circulating in the market has dyeing problem,with scarlet 808 as the dye.The TLC,HPLC and HPLC-MS methods established in this article can quickly screen and confirm the scarlet 808 in cinnabar(elutriation).

發文機構：Chongqing Institute for Food and Drug Control

關鍵詞：Cinnabar(elutriation)DYEINGHighperformanceliquidchromatography-massspectrometry

分類號： R28[醫藥衛生—中藥學]